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By; น.ส. ภควัทคีตา ภูริบัญชา ID 4812610139

subject;Human Genetic (พันธุศาสตร์มนุษย์)

นักศึกษาคณะสหเวชศาสตร์ ชั้นปี 3

มหาวิทยาลัยธรรมศาสตร์

Assignment 1

Gene sequence searching
: LEP leptin (obesity homolog, mouse) [ Homo sapiens ]
GeneID: 3952 updated 03-Jun-2007
Official Symbol
LEPprovided by HGNC
Official Full Name
Leptin (obesity homolog, mouse)provided by HGNC
Primary source
HGNC:6553
See related
HPRD:01249; MIM:164160
Gene type
Protein coding
RefSeq status
Reviewed
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
OB; OBS
Summary
This gene is similar to the mouse obesity gene (ob). The protein encoded by this gene is secreted by white adipocytes. In the mouse study, mutations in this gene are linked to severe and morbid obesity.
Genomic regions, transcripts, and products
Go to reference sequence details
Genomic context
chromosome: 7; Location: 7q31.3See LEP in MapViewer
Bibliography
Related Articles in PubMed
PubMed links
GeneRIFs: Gene References Into FunctionWhat's a GeneRIF?
General gene information
Markers
OBS(e-PCR)
Links: UniSTS:35280
Alternate names: GDB:455256; sWSS2619
STS-U43653(e-PCR)
Links: UniSTS:35281
Alternate names: RH75868; sts-U43653
RH70631(e-PCR)
Links: UniSTS:78152
Alternate name: U43653
LEP-3(e-PCR)
Links: UniSTS:253879
D8S2279(e-PCR)
Links: UniSTS:473907
Alternate name: GDB:1323207
NoName(e-PCR)
Links: UniSTS:481242
Phenotypes
Obesity, morbid, with hypogonadism
MIM: 164160
Obesity, severe, due to leptin deficiency
MIM: 164160
Pathways
KEGG pathway: Adipocytokine signaling pathway
04920
KEGG pathway: Cytokine-cytokine receptor interaction
04060
KEGG pathway: Jak-STAT signaling pathway
04630
KEGG pathway: Neuroactive ligand-receptor interaction
04080

Homology
Mouse, Rat
Map Viewer
GeneOntology Provided by GOA
General protein information
Names
leptin
obesity
leptin (murine obesity homolog)
obesity (murine homolog, leptin)
LEP
Official Symbol LEP and Name: leptin (obesity homolog, mouse) [Homo sapiens] Other Aliases: OB, OBSOther Designations: leptin; leptin (murine obesity homolog); obesity; obesity (murine homolog, leptin)Chromosome: 7; Location: 7q31.3Annotation: Chromosome 7, NC_000007.12 (127668566..127684916)MIM: 164160GeneID: 3952

DR004277
Reports
TC120021 Human placenta, large insert, pCMV expression library Homo sapiens cDNA clone TC120021 5- similar to Homo sapiens leptin (obesity homolog, mouse) (LEP), mRNA sequencegi66264150gbDR004277.1[66264150]


GenBank file
1: DR004277. TC120021 Human pl...[gi:66264150]
LOCUS DR004277 681 bp mRNA linear EST 17-MAY-2005
DEFINITION TC120021 Human placenta, large insert, pCMV expression library Homo
sapiens cDNA clone TC120021 5' similar to Homo sapiens leptin
(obesity homolog, mouse) (LEP), mRNA sequence.
ACCESSION DR004277
VERSION DR004277.1 GI:66264150
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 681)
AUTHORS Birkett,C., Cho,J., Gau,Y., Hamer,R., Kelly,S., Kovacs,K., Liu,L.,
Liu,X., Porter,J., Sachs,A., Shu,Y., Sun,Z., Wong,J., Wu,M., Zhang
,X., Jay,G. and He,W.
TITLE High-throughput cloning of full-length human cDNAs directly from
cDNA libraries optimized for large and rare transcripts
JOURNAL Unpublished (2005)
COMMENT Contact: Kovacs, KF
High Throughput cDNA Cloning
OriGene Technologies, Inc. ( www.origene.com )
6 Taft Court, Suite 100, Rockville, MD 20850, USA
Tel: 301 340 3188
Fax: 301 340 8606
Email: cDNA@origene.com
This EST submission is part of an on-going human full-length
cloning project at OriGene Technologies, Inc.
Please contact OriGene for access.
OriGene Technologies, Inc.
6 Taft Ct. Suite 100
Rockville, MD 20850
Tel: (301) 340-3188
http://www.origene.com
Seq primer: pCMV6 5prime forward vector primer, OriGene
Technologies Inc.
FEATURES Location/Qualifiers
source 1..681
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="TC120021"
/tissue_type="Placenta"
/clone_lib="Human placenta, large insert, pCMV expression
library"
/note="Vector: pCMV6-XL4; Site_1: EcoR1; Site_2: Xho1/Sal1
compatible end ligatio; Oligo-dT primed reverse
transcription optimized for large and GC rich mRNA
transcripts, cDNA size selection, optimized ligation for
large inserts into mammalian expression vector, random
clones selected for end sequence verification of
full-length genes"
ORIGIN
1 cacgaggttg caaggcccaa aaagcccatc ctgggaagga aaatgcttgg ggaaccctgt
61 gcggattctt gtggctttgg ccctatcttt tctatgtcca agctgtgccc atccaaaaag
121 tccaagatga caccaaaacc ctcatcaaga caattgtcac caggatcaat gacatttcac
181 acacgcagtc agtctcctcc aaacagaaag tcaccggttt ggacttcatt cctgggctcc
241 accccatcct gaccttatcc aagatggacc agacactggc agtctaccaa cagatcctca
301 ccagtatgcc ttccagaaac gtgatccaaa tatccaacga cctggagaac ctccgggatc
361 ttcttcacgt gctggccttc tctaagagct gccacttgcc ctgggccagt ggcctggaga
421 ccttggacag cctggggggt gtcctggaag cttcaggcta ctccacagag gtggtggccc
481 tgagcaggct gcaggggtct ctgcaggaca tgctgtggca gctggacctc agccctgggt
541 gctgaggcct tgaaggtcac tcttcctgca aggactacgt taagggaagg aactctggct
601 tccaggtatc tccaggattg aagagcattg catggacacc ccttatccag gactctgtca
661 atttccctga ctcctctaag c

FASTA file
1: DR004277. TC120021 Human pl...[gi:66264150]
>gi66264150gbDR004277.1DR004277 TC120021 Human placenta, large insert, pCMV expression library Homo sapiens cDNA clone TC120021 5' similar to Homo sapiens leptin (obesity homolog, mouse) (LEP), mRNA sequence
CACGAGGTTGCAAGGCCCAAAAAGCCCATCCTGGGAAGGAAAATGCTTGGGGAACCCTGTGCGGATTCTT
GTGGCTTTGGCCCTATCTTTTCTATGTCCAAGCTGTGCCCATCCAAAAAGTCCAAGATGACACCAAAACC
CTCATCAAGACAATTGTCACCAGGATCAATGACATTTCACACACGCAGTCAGTCTCCTCCAAACAGAAAG
TCACCGGTTTGGACTTCATTCCTGGGCTCCACCCCATCCTGACCTTATCCAAGATGGACCAGACACTGGC
AGTCTACCAACAGATCCTCACCAGTATGCCTTCCAGAAACGTGATCCAAATATCCAACGACCTGGAGAAC
CTCCGGGATCTTCTTCACGTGCTGGCCTTCTCTAAGAGCTGCCACTTGCCCTGGGCCAGTGGCCTGGAGA
CCTTGGACAGCCTGGGGGGTGTCCTGGAAGCTTCAGGCTACTCCACAGAGGTGGTGGCCCTGAGCAGGCT
GCAGGGGTCTCTGCAGGACATGCTGTGGCAGCTGGACCTCAGCCCTGGGTGCTGAGGCCTTGAAGGTCAC
TCTTCCTGCAAGGACTACGTTAAGGGAAGGAACTCTGGCTTCCAGGTATCTCCAGGATTGAAGAGCATTG
CATGGACACCCCTTATCCAGGACTCTGTCAATTTCCCTGACTCCTCTAAGC

Genomic
NC_000007.12 Reference assembly
Range
127668567..127684917
Download
GenBank FASTA
GenBank file
1: NC_000007. Reports

Homo sapiens chro...[gi:89161213]


Links
· Comment
· Features
· Sequence
LOCUS NC_000007 16351 bp DNA linear CON 30-AUG-2006
DEFINITION Homo sapiens chromosome 7, reference assembly, complete sequence.
ACCESSION NC_000007 REGION: 127668567..127684917
VERSION NC_000007.12 GI:89161213
PROJECT GenomeProject:168
KEYWORDS HTG.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 16351)
AUTHORS International Human Genome Sequencing Consortium.
TITLE Finishing the euchromatic sequence of the human genome
JOURNAL Nature 431 (7011), 931-945 (2004)
PUBMED 15496913
REFERENCE 2 (bases 1 to 16351)
AUTHORS Hillier LW, Fulton RS, Fulton LA, Graves TA, Pepin KH,
Wagner-McPherson C, Layman D, Maas J, Jaeger S, Walker R, Wylie K,
Sekhon M, Becker MC, O'Laughlin MD, Schaller ME, Fewell GA,
Delehaunty KD, Miner TL, Nash WE, Cordes M, Du H, Sun H, Edwards J,
Bradshaw-Cordum H, Ali J, Andrews S, Isak A, Vanbrunt A, Nguyen C,
Du F, Lamar B, Courtney L, Kalicki J, Ozersky P, Bielicki L, Scott
K, Holmes A, Harkins R, Harris A, Strong CM, Hou S, Tomlinson C,
Dauphin-Kohlberg S, Kozlowicz-Reilly A, Leonard S, Rohlfing T, Rock
SM, Tin-Wollam AM, Abbott A, Minx P, Maupin R, Strowmatt C,
Latreille P, Miller N, Johnson D, Murray J, Woessner JP, Wendl MC,
Yang SP, Schultz BR, Wallis JW, Spieth J, Bieri TA, Nelson JO,
Berkowicz N, Wohldmann PE, Cook LL, Hickenbotham MT, Eldred J,
Williams D, Bedell JA, Mardis ER, Clifton SW, Chissoe SL, Marra MA,
Raymond C, Haugen E, Gillett W, Zhou Y, James R, Phelps K, Iadanoto
S, Bubb K, Simms E, Levy R, Clendenning J, Kaul R, Kent WJ, Furey
TS, Baertsch RA, Brent MR, Keibler E, Flicek P, Bork P, Suyama M,
Bailey JA, Portnoy ME, Torrents D, Chinwalla AT, Gish WR, Eddy SR,
McPherson JD, Olson MV, Eichler EE, Green ED, Waterston RH and
Wilson RK.
TITLE The DNA sequence of human chromosome 7
JOURNAL Nature 424 (6945), 157-164 (2003)
PUBMED 12853948
COMMENT GENOME ANNOTATION REFSEQ: Features on this sequence have been
produced for build 36 version 2 of the NCBI's genome annotation
[see documentation].
On Mar 3, 2006 this sequence version replaced gi:51511723.
The DNA sequence is part of the fourth release of the finished
human reference genome. It was assembled from individual clone
sequences by the Human Genome Sequencing Consortium in consultation
with NCBI staff.
FEATURES Location/Qualifiers
source 1..16351
/organism="Homo sapiens"
/mol_type="genomic DNA"
/db_xref="taxon:9606"
/chromosome="7"
STS 13519..13768
/standard_name="STS-U43653"
/db_xref="UniSTS:35281"
STS 13519..13624
/standard_name="OBS"
/db_xref="UniSTS:35280"
STS 13628..13823
/standard_name="RH70631"
/db_xref="UniSTS:78152"
gene 1..16351
/gene="LEP"
/note="Derived by automated computational analysis using
gene prediction method: BestRefseq. Supporting evidence
includes similarity to: 1 mRNA"
/db_xref="GeneID:3952"
/db_xref="HGNC:6553"
mRNA join(1..29,10714..10885,13127..16351)
/gene="LEP"
/product="leptin (obesity homolog, mouse)"
/exception="unclassified transcription discrepancy"
/note="Derived by automated computational analysis using
gene prediction method: BestRefseq. Supporting evidence
includes similarity to: 1 mRNA"
/transcript_id="NM_000230.1"
/db_xref="GI:4557714"
/db_xref="GeneID:3952"
/db_xref="HGNC:6553"
CDS join(10742..10885,13127..13486)
/gene="LEP"
/GO_component="extracellular region; extracellular space
[pmid 9054940]"
/GO_function="hormone activity"
/GO_process="cell-cell signaling; energy reserve
metabolism [pmid 9537324]; signal transduction"
/note="leptin (murine obesity homolog); obesity (murine
homolog, leptin)"
/codon_start=1
/product="leptin precursor"
/protein_id="NP_000221.1"
/db_xref="GI:4557715"
/db_xref="CCDS:CCDS5800.1"
/db_xref="GeneID:3952"
/db_xref="HGNC:6553"
ORIGIN
1 gtaggaatcg cagcgccagc ggttgcaagg taaggccccg gcgcgctcct tcctccttct
61 ctgctggtct ttcttggcag gccacagggc cccacacaac tctggatccc ggggaaactg
121 agtcaggagg gatgcagggc ggatggctta gttctggact atgatagctt tgtaccgagt
181 tctagccaga tagaaggtta ccgggagctg gggagcgttg gatttgctgc tgggctgtgc
241 cggtgcccag aaggcaggac cttgcagaac cagccaggtc cctgggagac tgtcagaccc
301 accaacctgg tggcattcgc agagctgaga tgcattggaa attgccttgg gcacatcccc
361 aaagatcagg atgtcccacc ccagtctgaa ggagataaag ttgggggtag gagagacgca
421 gatgcaagtg atcagtctca gtcccagaca ttgccttgct ctgcgggtag gaattcagga
481 ttcattttcc agggaagttc ctgacctctg aatgagaggg gctgtgtaag gccaatgcct
541 gggaggaagg caaggatgag tagaggtggg gggaaacaag tgtcaggaag actcaaaatc
601 ttccagagaa attgtgcagg gtcttaccag atctgtcctc aaagccatgc aaattgcctt
661 ctttgcaatg catacaatga ggtgtctctg ggggtcagaa ctggttatta gggaacttct
721 agccaggact gctaaatacg cgctgttggc ccaccaggct cacctatagc cttccttcag
781 tctgggcttg gtttggattt cactgtgggt gccatcgcct ttacactcct gtttctatag
841 tttaaagata gtggtgcttt gggaaagtga ctccttaaat acagttaggt ccaagtgaga
901 caagtggcct ggctgtcatt tcagaatagc agcttccaag aggtgattaa tttctgttgg
961 aagggtgatc tttggggagg tgggtgaaga gcagagactt ggtggtaccg ttccaggagc
1021 acaggctctc ttcctttgca gtgcagaatg acctctggca gccggagttg tgtttgttct
1081 gtaggattct gaggtgggcc atgggcagct ggaactgggg aattttgcca atctctttca
1141 tattaggatt gtctgcagaa ccagatatgg aggcttctag caacgtgagt gctcctgttc
1201 taatgccctt agaaacaaga aggccacact gatcatttct ctcacttagg cagggagaca
1261 aggcaagaga gaaacagtgg atgcttttag gttctttccc ttcccaagca gttgtggaca
1321 ttgggctgag gggaacattt ccacattggc taaaggagcg tcctcctcat attttgtaca
1381 ttttataccc aaaataactc ttcttggtat ttggggaaat attttcctcc ccgtccattc
1441 caggaaatgg ctccaagtgc caaggacaga gccagggaag ttgcaatgaa ttcctgcccg
1501 tcagccccag gcagatgcct tgcacgtctg agtggcccat gcagagcgtg gaggtggccg
1561 ccacggaacc tgggtcaatg tcccaccccc gcttagatgc caccaggggc gtgggagcca
1621 aggagagaag aggggctcca ggaaggtaga gtccttgtgt cttgtgcatc tgtgaacagc
1681 actggtatga tttaaaggaa aattgagcca aattttccgg cagtcagtta ccccatcccc
1741 accggggtag gagtctggca gccgcagctc cattctggcc agtcggcaga gagccttgaa
1801 attcttcttt gtccacacag ttgtctcaga gaaacagaga ggttgtttct gcttaaaaac
1861 aacacacttg gtgtctgggc ccacagactc ctttgcactt attccacgtg tgacagccaa
1921 tgtgcctcgt tgcttagcag acagcatgtt accgtctttc ctgctcagtt tgttagctct
1981 atggaatgga atttataatc aatgcccata ccaacatttc actaatatca taggagattt
2041 agtctccatc tgggtgtaca ttacatttgc tctggggtgc tccaggctgg ggggttgcca
2101 aggaagagaa gagaaaccgc agagaagacg ggagggcagg gcaggggtct ctgagaaggg
2161 gaggggtccc agagtgcagg agcaggagcc aggctcatga aaggggccac gggcgggagt
2221 atccagggac ggcagtcaag atggagcaca gcttaggaag ctgaagggaa tcctggccca
2281 cctgggtgct agagggcaca taggaagtgc aggaagcaga ccaaggtccc caagagaggg
2341 agacctggac gctgaagcat tttctgtctt tattaagaca actccgtaag aattcctgct
2401 gggccaaagt gaattctagg atgcgacttt aagatgggag caagcgaacc attgaggagg
2461 caggttaccc tagttagcca atgcagatcg agaatgggaa atctttcatt tattcatgca
2521 acagatattt aacgaagccc tgccgtgttc caggcctgtg atagatgctg gaacaggtac
2581 agagatacag gtgtcattaa ttgatcaggg caacctctcc ttctgagtct tgctggagct
2641 tcagatgccc ctcacacaga gctcgaggga gcctcaacaa ttgatcagaa gtcaggcacc
2701 atggctcacg catataatcc cagcactttg ggaggccaag gcaggtggat cactggagcc
2761 caggagttcc agatcagctg gggcaacatg gcaaaacccc atctctatta aaaaaaaaaa
2821 aagtaactgg atgtgatggt acacacctgt agtcccagct acttgggagg ctgagaggtg
2881 ggagaattgc ttgagcccgg gaagtcgggg gtccagtgag ccttgatcac accactgcac
2941 tccagcctga gtgacagagc aagaccctga cacacacaca cacacacaca cacacacaca
3001 cacagattag agctgaaaca ggagtagaaa cctatctgta tctctgatga gatcagatct
3061 ttctgatgaa cagaaagaat gtaacccctg tactcacacc ctctctgctg gttacatatg
3121 ttaacacgat ttctcaaatg aggcttttgg ttgcaaataa gagaaaatca ctcacgctgg
3181 ccctgtgttt ttcaaattgt ttattgtgat caacatttga aaaaagagcc gagactctca
3241 agagtgcatt acccacggta agggtgaatt ttacttcttg acacttattt ctcttacatg
3301 tatctatctg tctcaaatga aaaatatatt tagaaagttg aaagctatcc aagtgagtat
3361 aagaaaagag tatctcaccc tgaaggctaa ggacagggag ggccaccagg cctcacgagg
3421 acccaggaac cacaaagaag gctaggaagg agcacaggcg gtgaccatac tctggctcag
3481 tggctatgtg ggctctggtc tctctcagct gttccatgca tatgaggcca aatgtggcta
3541 ccctagagct tctgagccct caacagagat gaactggact ctctgcagcc ccactctaaa
3601 ttcctaagag agaagttgat tgacccaatc agggtcagga gaaggaaggg aggaggaaag
3661 ggaggagaga agagcctctt cgtctcttgc ctaccactgg ccaggcaatt gtagccaagg
3721 gggctggagt gtaaatgcaa acatagccat caagggttgt gtatgtgtgt gtgtgtgtct
3781 gtgtgtgtgt atgtgtgtct cttgggtagg ttagatctcc caggaggtcc ctactaaaca
3841 gacttaagcc cgcaaaattt tagctctcca gcctcacaca ctccacccct ctaccatatt
3901 gaatcttccc aaaccaacta tggctttccc taactccgga gcttggcctg gaatgccctg
3961 cttcccctct ttcccctggg gaacgcctgt ccttcaggcc tcagttcaca cactgcctcc
4021 cttgcaaagc tctcctccca tccccggagt ccctcttccc ctttgttctt tgggttctat
4081 gcttcttccc tcataactcc caccaggttg tgttaaaatg agttgttcaa ggtcctgtct
4141 gttccactag attctgagca acttggagaa cgaagatcca aacttcgctg cctttatttc
4201 ctcctttgtt cttttctcat ccccaagtcc cttccaactt ggagttatga agaaaggaag
4261 gaaggaaggg tgggagggaa gaacaggagg ggatcccaca ggagaatgtg tatagggaga
4321 ggactcagac tagctaaagc ttttccctca taattaatag caaataccat gttacctgaa
4381 tttaattcac agtagcatac aaaagactcg ctttgttctc cccattgatg tcatcagagg
4441 gctgtgggca ggcctaatct tggctcagga ggccctccag cctggatcta aagagcagca
4501 gatgggccag gctcggtggc tcatgcctgt aatcccagca ttttgggagg ccgaggcggg
4561 tggatcacga ggtcaggagt ttgagaccag cctggccaag atggtgaagc ctcgtctcta
4621 ctaaaaatac aaaaattagc caggtgcggt ggtgggcgcc tgtatttcca gctacccggg
4681 aggctgagga ggctgaggca ggagaatcgc ttgaacccgg gaggcggagg ttgcagtgag
4741 ccgaggtcac gccactgcac tctagcctgg gcaacagagc aagactccgt caaaaaaaaa
4801 ataaaaaaat aaaaaaataa aaaaaataaa gaggagcaca catctctgcc catcctaact
4861 cccactttga cattgaggtc cccaggatgg agggtctgcc tccatctgcc ttgtcccctg
4921 caatggtggg aaggtgatgg agctcaagtc tagaggccac cagcttctta gggaggtagg
4981 aggtggaggg tggggtgcgg gccctgcaca caactgccaa gtgaggatgg gggtggggtc
5041 cacctgagga taagtaacag tgaggctggt gcagaggacc caggtggagg tagacagcag
5101 aatttgtggt ggggtggatg gcacattata taagcctctc ttgctgccct gtttactgag
5161 attgtttcat tatctttttt ggcttttgtt tttaagagat ggggtcttgc tgtgtcacac
5221 aggctggagt gcactgtgtg atcatacctc actgcagcct cgacatcctg ggctcaggca
5281 aacctcccac cttggcctcc caagtagctg ggaccacaag cgtttgccac cacactcagc
5341 tatttttatt tttattttta ttttttttag agatggggtc ttgctgtgtc gcccaggctg
5401 gtcttgaact cctgggctca agcgatcctc ctgccttggc ctcccaaagc cctgggatta
5461 taggctgagc caccacaccc agccacattt catctgtgca gctccagggg ctccacattc
5521 tactcttctc atttcttctc cagggtaccc atggcaaggg atgagggtag aagatggggc
5581 agccaggcct tgattaaagg agaaggaagg cagcctgtgg agagggcagc ccagggagtg
5641 cagagagaag tgggccatga gggagacagc agagtgcagg ctgcgtccca aatgagcata
5701 cagcccactg tgagcccacc atcttcctag agacccctct cctctccagg agctgcttca
5761 gtagcactca gaggaaagaa tgatgctgta tcaacatttc agcagctcat cttttaactc
5821 taagaaaatg gcagctccta aatgttcaaa actgctttgg aaacttctgg agagaggttt
5881 tgcagctcag gcagacagct gatcgcggcc tttcttccac cccaacccat gctctcccca
5941 tgctctcctg ccacagctgc agcgggcccc tgggtcctac atttgcagcc ctttgtctct
6001 gagctcagac ttccaattcc aagcggcagc tgggcaggct caccagcatg tccagccagt
6061 actaggacat cagcaggagc ccaaccacct ctttccaaaa tctctcctca tgtctctcct
6121 agtttccatc tccatccttc tagtcagcca ggctgaaaac atttgctcct cagggtgcag
6181 aagggaaagc tttgcctccc ttcctggtgc tcactgcccc tgcgattcca gcccaagccc
6241 tccccggctc ctcaccctgg tgtcagctgg aagccaccat ctcctaaacc cacctgtgtt
6301 cttccacctc tgccagggct gccctctcct ccaccttcac aaactcaatt cctacccatt
6361 ctcaggtccc ttatcaaatg ccatctcctc catgatgcct ccctgattcc cctgctggaa
6421 ataatggtga taacagctaa ggcattgggg ttggctacgt gccaggcaag gagttggcac
6481 tttacatgct ttatctcatt tcagccacat aacatcgaca ggtggcatta tgattcatat
6541 catccccatc tgatagccag gaaaactgag tcccagagag gttagccact ttcctagggc
6601 cctgtgctct gactcaagca tagctctgag gaactctagc attcatcagt ttaagcacca
6661 tgactttctt tgctgagtca cccaaggcat ttcttcattt aaatgttctt ccttggccag
6721 gcgcagtggc tcaggcccaa tgcggtggct cacgcctgta atctcaacac tttgggaggc
6781 cgaggtgggc agataatctg aggtcaggag ttcaagacca gcctggccaa catggtgaaa
6841 ccccatctct actaaaaata caaaaaaatg aggctgggcg tgatgactca cacctgtaat
6901 cccagcactt tgggaggcca aggcaggtgg attacatgag gtcaggagtt cgagaccagc
6961 ctggccaaca tggtgaaatc ctatctctat taaaaataca aaaaattagc caggcatggt
7021 ggcaggcacc tgtaatccca gctacttggg aggctgaggc aggaaaatgg cttgaacccg
7081 ggaggtggag gttgcagtga gccaaggttg caccattgca ctccagcctg ggcaaaaaga
7141 gggaaacatc gtctaaaaaa gaaaaaaaaa aaattagcca ggctgggtgg tgcatgcccg
7201 taattccagc tactcaggag gatgaagcaa gagaattgct tgaacccagg aggcagagat
7261 tacagtgagc tgagatcaca acactgcact ccagcctagg taaagaacaa gactccatct
7321 caaaaataaa taaataaaaa taaatgttct tccttgcaat gaagttaaat atgtaaattc
7381 tcaaaccagt tgcttaaggg cacagttttg ttctttacct atatttttaa caaatatttt
7441 atgtaagtag ttgacaaaat caaatactgt gtacactacc gaggcttccc tgggaaagcc
7501 atcagcctct gccccatccc ttcccactcc tgattccact ttcctgtgtt tccatatctt
7561 tttcatgtct gtttctggcc cacagtgggc gatcaataca tgttagccac caaccatcaa
7621 acctatattg agtaattatg gtatgtcagg cactatgctc aatgaaattg tattaggctt
7681 gtacaaaagt aattgtggtt tttaagagta atggcaaaaa cggcagttac tttcgcacca
7741 actatttgct gccttgaatt attcctcctc tcctcatccc taaaccctgc tcctcccagc
7801 cattcttcct ccccttcttg ggccatggcc aggccccacc caggtactaa gactcaggtg
7861 aaccaaggaa gacttaatgc ccactctttt ctgatgccca tgttggcatg tgttaagtcg
7921 gttagcatta agtttggctg catttagcag agacccaaaa gaacagtgcc ttttaaaagg
7981 cagaggttat gtctctcaca cacacccagc acaagtccaa gaccagcatg gcatctcagc
8041 tccatcaacc tcaggaaccg agctcctgca gctccctgcc ctgcagttga taaggtgagg
8101 tctttgtcct cctggttcaa gatggtgcta gaatgttggc taccatatct atagtccagg
8161 catcagaatg gagcaaggga tgaaaaagga agagatgaag gcacacgaca ggttcctgag
8221 agctggcaca ggacacttct gcttatattt cactggccag aacttagtca catggtcaca
8281 cctagttggg agactctgag aagtaaagta tttattctag atggccatat ccctacctaa
8341 gacttggagt tttctatgac tggggaagaa cggaagacaa gatattggga aagactagca
8401 gcctctacta aaagggtgat ctgtgttgat gtgcgtgtgt gtgtgatgtt tgtatgagca
8461 tgtgtgttat gtgttgtgtg ttggtggggc agattcttgc gagcactttg gtctcagatg
8521 gacctgctac cagttctctc tgcagacccc cataggtttc tcctaaacct ggcctctcct
8581 attaggcagc cttactcagc ggcagcttct cagctccatg ttttcaagga accacaattt
8641 atttccagca tccactgaag catattatca gtggtgatag agggggcttg taaaactgtt
8701 tttccactta ggtattagag ggtggccatt atttgagagt gactatgacc acagttaatc
8761 tggtaataaa ttctcttggg taggaggaaa ggaaaggatg ctttaaggaa gcatcttgcc
8821 aggagacaca aagctaacaa gagtggagcc tgcagctgga gccgcagagc ctaatcacta
8881 cacccgccca tctctgctag ggtttcatga cttcgtatcg gggattagca gtatttaact
8941 ctgttgcaca aacatttggt gtattattca ggtaacaagt agctaataga ggaagtttta
9001 cttttttaag acataaattt gccttttccc aaattacttg gtacatagta cttttcatgt
9061 ttgaagttga gatgtgggta caataccata gctttattcc agagcagggt atttgtttcc
9121 aaatgccatg ttcccagcag ctgcccttga ctgggaattg gggtgtgatt tgggcttttc
9181 cttaaatcct tgaggagctg gaggggtggg tggctcgcac tcctgctttc tggatctgaa
9241 tcctgactct gtcatggacc tgtttgactt tgggcaagtt gactcctatt cctgagcccc
9301 atatttttct cttctgtgaa attcagatta aaaaaacatg gctttgatca aacattataa
9361 ataatatata gacagactgc ttgtttttat tgtattgcca gaaatgaatc ctactaatat
9421 tgccatctat ggacagaaaa tgtattacct gtcttcatca agacccagac gaggaagaac
9481 acgaaaagcg gagattaatt ttactgccat ctccagaacc gtcatcctaa tatttactta
9541 cattttatta ttatttcagg ctcatgcaca tatacttagc atggatcatt ggccacagac
9601 tcgcatacat ttaactttat taccttttgc ctcatgtatc tcattaaaat tttgctgctt
9661 aatcaaggat ctgcatatta ttttaatttt agaattcaca gttccaagac tttgaaagtt
9721 tcaagcgttc tgggtgaatg tgttatgctc tctcccgcca ccatgtcttt ataccccctg
9781 atttctcagc cactatggca accactttct actcttagta gcccatattt agtccaatcc
9841 ccagctcagg agacacttct tccagggagc cccctgtgcc ttccagtagt atcttgtacc
9901 tgcccttttt gcaaagctct ttcctcctgg cttagaatgg cccattgacc tgtttgtttc
9961 tcctattaaa ctgtaagcca ctcgagggta gagagcatct gttgttcacc attgcatcct
10021 cggtgctgag cactgcgtct gacatattat ttagaaggtc agtaagtgct agtgggattc
10081 aggctcccag tgggtgggag agaaaggacg taaggaagca agtggtaaag gccctcacag
10141 agtatcagca ggctggtgtg agggagaaat gcagaggatg ggtgagtagc ataatcgcta
10201 atgatagggt aatgatagag cacatttcac aacaccttta agccctttca cgtgcatcag
10261 ataatttgat cctcataaaa gcctagagat agatatatta cagggatgaa ggtggagtat
10321 tttgtggtta tgtgatatgt ttaaaattat gcagtgagta aatgactggg ttcaaaccag
10381 accttaaaag tctgttatct ttccctcgag catgcaatga agtctacatc atccctacca
10441 tgtccatttg atcacaccct ggcctcacag ctctgtggtc tacaggatac ctcatggtgg
10501 ttttattgac cagacaataa tcctctttct aaggggatgc atttcattaa tacatatgta
10561 gatcatgaat tgtctttgac tttgagggga tggtagccag agcagaaagc aaagctgatt
10621 ttcatccccg tctggtaatg tggttggtaa tgtgaagatg ggtgtattct gagataccgg
10681 ctccttgcag tgtgtggttc cttctgtttt caggcccaag aagcccatcc tgggaaggaa
10741 aatgcattgg ggaaccctgt gcggattctt gtggctttgg ccctatcttt tctatgtcca
10801 agctgtgccc atccaaaaag tccaagatga caccaaaacc ctcatcaaga caattgtcac
10861 caggatcaat gacatttcac acacggtaag gagagtatgc ggggacaaag tagaactgca
10921 gccagcccag cactggctcc tagtggcact ggacccagat agtccaagaa acatttattg
10981 aacgcctcct gaatgccagg cacctactgg aagctgagaa ggatttgaaa gcacagggct
11041 ccactctttc tggttgtttc ttttggcccc tctgcctgct gagattccag gggttagtgg
11101 ttctaattct aaaccactcc aagaacattt gattttgcta catgtttcca tttaaaaatc
11161 ataggatttg ggctgggtgt ggtggcttgt acctgtcatc ccagcacttt gggaggccaa
11221 agcaggagga tcattcgagc ccaagagttc gagaccagcc tgggcagcat agggagaccc
11281 catctctaca aaaataataa aaaatgttag ctgggcatgg tggtgtgtac ctgtggtccc
11341 agctagggga ggctgagatg gaaggatcac ctgagcctgg gaggttgagg ctgcagtggg
11401 ccctgatcat gccaccgtgc tccagcctgg gtgacagagt gagaccttgt ctcaaaataa
11461 ataaataaat aaataaaagt cataggattt gatcaggcat gatgggtcac atctgtaagc
11521 ccattgcttt aggaggccaa ggtaggagga tcagttgagg ccaggagttc aagaccagcc
11581 tgggcaacat ggcaagacct ctctctctaa tttttaaaaa aataaaaatt aaagataaga
11641 aaaaaatcat aggattctca tgaggcctca cgtgcttatt ttcaacctac caaggggaaa
11701 cccaggcctc agcgattagc tgagccacat gcaggcacag ccactgtctc tttccttcct
11761 gtcccctctg tccccacctt ctgcgctcgc cttcctccct gacttcactt ccttgaatct
11821 tagtgcctac gaccagaggg agctgtgaag ttccttgtgt cccattggca ggaacaagac
11881 ccccagaagc atctcctcag ggcctctatc ccatctctag atgtgcttgt cattagggtt
11941 cttgtagttc cagctgatct ctggccctgc cgctcaaaga tacccaaaag agcgagtcta
12001 ccctttttca cattcaaccc tctactgatt tgcaaatagc agtcagtgcc caccctggtc
12061 ttttctctgg ggtccagcag gcctagacct tcagccattt tcctgatgag gtctgtattt
12121 gaaattagga agattaagtt tgaatcttca cacttctgat gtctgtgaga tcttcagcaa
12181 gttccttact gtctttaagc cttgttttca tcatctggat aatggggata tcacacacta
12241 ttcacaaggt tgttatgagg cctaaattag ctaaagcaat tgaatcctcc ttaccccctg
12301 catggagctc tctggagact tccacgtctc ctggtcattg tgggtgtctt atggtagtct
12361 tgggcagtta gggagaagtt aggtgtctgg aagcaaagat ggctcagaac tagatagagt
12421 cttgggcatt ttatagataa aaactcttgt ctcctttaaa aataataaaa aaaaattagc
12481 tgggcatatt agccactcag caagactgca cgtgatagat cccgagtgcc ccaccttggg
12541 tggtgtaata cacaatatca cgggagcccc gggtagtaac cacggaggtg tcagcctcag
12601 tgctgtgggc agatggatgg ggagagcctc ccggaactgg agtcactgga gcagggttgg
12661 ggggcctcac tgagggtacg gccttgatct ctaaggagga gggactgcct ggaaaagctg
12721 actgggaggg aggactcggc tgggggtaga agggactagg gaaggctggg ggtgggggtg
12781 cttatggagg acctcagatg cctggggaac agactccact aaataaaaca tatgaaacca
12841 tggctggttc ttcagcagag gccatgtaga gaaaggaatg acctaggaaa gttggcctgg
12901 aagtggaggg aaggatggtg tgggaaaagc aggaatctcg gagaccagct tagaggcttg
12961 gcagtcacct gggtgcagga tacaagggcc tgagccaaag tggtgaggga gggtggaagg
13021 aggcagccca gagaatgacc ctccatgccc acggggaagg cagagggctc tgagagcgat
13081 tcctcccaca tgctgagcac ttgttctccc tcttcctcct gcatagcagt cagtctcctc
13141 caaacagaaa gtcaccggtt tggacttcat tcctgggctc caccccatcc tgaccttatc
13201 caagatggac cagacactgg cagtctacca acagatcctc accagtatgc cttccagaaa
13261 cgtgatccaa atatccaacg acctggagaa cctccgggat cttcttcacg tgctggcctt
13321 ctctaagagc tgccacttgc cctgggccag tggcctggag accttggaca gcctgggggg
13381 tgtcctggaa gcttcaggct actccacaga ggtggtggcc ctgagcaggc tgcaggggtc
13441 tctgcaggac atgctgtggc agctggacct cagccctggg tgctgaggcc ttgaaggtca
13501 ctcttcctgc aaggactacg ttaagggaag gaactctggc ttccaggtat ctccaggatt
13561 gaagagcatt gcatggacac cccttatcca ggactctgtc aatttccctg actcctctaa
13621 gccactcttc caaaggcata agaccctaag cctccttttg cttgaaacca aagatatata
13681 cacaggatcc tattctcacc aggaaggggg tccacccagc aaagagtggg ctgcatctgg
13741 gattcccacc aaggtcttca gccatcaaca agagttgtct tgtcccctct tgacccatct
13801 ccccctcact gaatgcctca atgtgaccag gggtgatttc agagagggca gaggggtagg
13861 cagagccttt ggatgaccag aacaaggttc cctctgagaa ttccaaggag ttccatgaag
13921 accacatcca cacacgcagg aactcccagc aacacaagct ggaagcacat gtttatttat
13981 tctgcatttt attctggatg gatttgaagc aaagcaccag cttctccagg ctctttgggg
14041 tcagccaggg ccaggggtct ccctggagtg cagtttccaa tcccatagat gggtctggct
14101 gagctgaacc cattttgagt gactcgaggg ttgggttcat ctgagcaaga gctggcaaag
14161 gtggctctcc agttagttct ctcgtaactg gtttcatttc tactgtgact gatgttacat
14221 cacagtgttt gcaatggtgt tgccctgagt ggatctccaa ggaccaggtt attttaaaaa
14281 gatttgtttt gtcaagtgtc atatgtaggt gtctgcaccc aggggtgggg aatgtttggg
14341 cagaagggag aaggatctag aatgtgtttt ctgaataaca tttgtgtggt gggttctttg
14401 gaaggagtga gatcattttc ttatcttctg caattgctta ggatgttttt catgaaaata
14461 gctctttcag gggggttgtg aggcctggcc aggcaccccc tggagagaag tttctggccc
14521 tggctgaccc caaagagcct ggagaagctg atgctttgct tcaaatccat ccagaataaa
14581 acgcaaaggg ctgaaagcca tttgttgggg cagtggtaag ctctggcttt ctccgactgc
14641 tagggagtgg tctttcctat catggagtga cggtcccaca ctggtgactg cgatcttcag
14701 agcaggggtc cttggtgtga ccctctgaat ggtccagggt tgatcacact ctgggtttat
14761 tacatggcag tgttcctatt tggggcttgc atgccaaatt gtagttcttg tctgattggc
14821 tcacccaagc aaggccaaaa ttaccaaaaa tcttgggggg tttttactcc agtggtgaag
14881 aaaactcctt tagcaggtgg tcctgagacc tgacaagcac tgctaggcga gtgccaggac
14941 tccccaggcc aggccaccag gatggccctt cccactggag gtcacattca ggaagatgaa
15001 agaggaggtt tggggtctgc caccatcctg ctgctgtgtt tttgctatca cacagtgggt
15061 ggtggatctg tccaaggaaa cttgaatcaa agcagttaac tttaagactg agcacctgct
15121 tcatgctcag ccctgactgg tgctataggc tggagaagct cacccaataa acattaagat
15181 tgaggcctgc cctcagggat cttgcattcc cagtggtcaa accgcactca cccatgtgcc
15241 aaggtggggt atttaccaca gcagctgaac agccaaatgc atggtgcagt tgacagcagg
15301 tgggaaatgg tatgagctga ggggggccgt gcccaggggc ccacagggaa ccctgcttgc
15361 actttgtaac atgtttactt ttcagggcat cttagcttct attatagcca catccctttg
15421 aaacaagata actgagaatt taaaaataag aaaatacata agaccataac agccaacagg
15481 tggcaggacc aggactatag cccaggtcct ctgataccca gagcattacg tgagccaggt
15541 aatgagggac tggaaccagg gagaccgagc gctttctgga aaagaggagt ttcgaggtag
15601 agtttgaagg aggtgaggga tgtgaattgc ctgcagagag aagcctgttt tgttggaagg
15661 tttggtgtgt ggagatgcag aggtaaaagt gtgagcagtg agttacagcg agaggcagag
15721 aaagaagaga caggagggca agggccatgc tgaagggacc ttgaagggta aagaagtttg
15781 atattaaagg agttaagagt agcaagttct agagaagagg ctggtgctgt ggccagggtg
15841 agagctgctc tggaaaatgt gacccagatc ctcacaacca cctaatcagg ctgaggtgtc
15901 ttaagccttt tgctcacaaa acctggcaca atggctaatt cccagagtgt gaaacttcct
15961 aagtataaat ggttgtctgt ttttgtaact taaaaaaaaa aaaaaaagtt tggccgggtg
16021 cggtggctca cgcctgtaat cccagcactt tgggaggcca aggtgggggg atcacaaggt
16081 cactagatgg cgagcatcct ggccaacatg gtgaaacccc gtctctacta aaaacacaaa
16141 agttagctga gcgtggtggc gggcgcctgt agtcccagcc actcgggagg ctgagacagg
16201 agaatcgctt aaacctggga ggcggagagt acagtgagcc aagatcgcgc cactgcactc
16261 cggcctgatg acagagcgag attccgtctt aaaaaaaaaa aaaaaaaagt ttgtttttaa
16321 aaaaatctaa ataaaataac tttgccccct g

FASTA file
1: NC_000007. Reports

Homo sapiens chro...[gi:89161213]


Links
>refNC_000007.12NC_000007:127668567-127684917 Homo sapiens chromosome 7, reference assembly, complete sequence
GTAGGAATCGCAGCGCCAGCGGTTGCAAGGTAAGGCCCCGGCGCGCTCCTTCCTCCTTCTCTGCTGGTCT
TTCTTGGCAGGCCACAGGGCCCCACACAACTCTGGATCCCGGGGAAACTGAGTCAGGAGGGATGCAGGGC
GGATGGCTTAGTTCTGGACTATGATAGCTTTGTACCGAGTTCTAGCCAGATAGAAGGTTACCGGGAGCTG
GGGAGCGTTGGATTTGCTGCTGGGCTGTGCCGGTGCCCAGAAGGCAGGACCTTGCAGAACCAGCCAGGTC
CCTGGGAGACTGTCAGACCCACCAACCTGGTGGCATTCGCAGAGCTGAGATGCATTGGAAATTGCCTTGG
GCACATCCCCAAAGATCAGGATGTCCCACCCCAGTCTGAAGGAGATAAAGTTGGGGGTAGGAGAGACGCA
GATGCAAGTGATCAGTCTCAGTCCCAGACATTGCCTTGCTCTGCGGGTAGGAATTCAGGATTCATTTTCC
AGGGAAGTTCCTGACCTCTGAATGAGAGGGGCTGTGTAAGGCCAATGCCTGGGAGGAAGGCAAGGATGAG
TAGAGGTGGGGGGAAACAAGTGTCAGGAAGACTCAAAATCTTCCAGAGAAATTGTGCAGGGTCTTACCAG
ATCTGTCCTCAAAGCCATGCAAATTGCCTTCTTTGCAATGCATACAATGAGGTGTCTCTGGGGGTCAGAA
CTGGTTATTAGGGAACTTCTAGCCAGGACTGCTAAATACGCGCTGTTGGCCCACCAGGCTCACCTATAGC
CTTCCTTCAGTCTGGGCTTGGTTTGGATTTCACTGTGGGTGCCATCGCCTTTACACTCCTGTTTCTATAG
TTTAAAGATAGTGGTGCTTTGGGAAAGTGACTCCTTAAATACAGTTAGGTCCAAGTGAGACAAGTGGCCT
GGCTGTCATTTCAGAATAGCAGCTTCCAAGAGGTGATTAATTTCTGTTGGAAGGGTGATCTTTGGGGAGG
TGGGTGAAGAGCAGAGACTTGGTGGTACCGTTCCAGGAGCACAGGCTCTCTTCCTTTGCAGTGCAGAATG
ACCTCTGGCAGCCGGAGTTGTGTTTGTTCTGTAGGATTCTGAGGTGGGCCATGGGCAGCTGGAACTGGGG
AATTTTGCCAATCTCTTTCATATTAGGATTGTCTGCAGAACCAGATATGGAGGCTTCTAGCAACGTGAGT
GCTCCTGTTCTAATGCCCTTAGAAACAAGAAGGCCACACTGATCATTTCTCTCACTTAGGCAGGGAGACA
AGGCAAGAGAGAAACAGTGGATGCTTTTAGGTTCTTTCCCTTCCCAAGCAGTTGTGGACATTGGGCTGAG
GGGAACATTTCCACATTGGCTAAAGGAGCGTCCTCCTCATATTTTGTACATTTTATACCCAAAATAACTC
TTCTTGGTATTTGGGGAAATATTTTCCTCCCCGTCCATTCCAGGAAATGGCTCCAAGTGCCAAGGACAGA
GCCAGGGAAGTTGCAATGAATTCCTGCCCGTCAGCCCCAGGCAGATGCCTTGCACGTCTGAGTGGCCCAT
GCAGAGCGTGGAGGTGGCCGCCACGGAACCTGGGTCAATGTCCCACCCCCGCTTAGATGCCACCAGGGGC
GTGGGAGCCAAGGAGAGAAGAGGGGCTCCAGGAAGGTAGAGTCCTTGTGTCTTGTGCATCTGTGAACAGC
ACTGGTATGATTTAAAGGAAAATTGAGCCAAATTTTCCGGCAGTCAGTTACCCCATCCCCACCGGGGTAG
GAGTCTGGCAGCCGCAGCTCCATTCTGGCCAGTCGGCAGAGAGCCTTGAAATTCTTCTTTGTCCACACAG
TTGTCTCAGAGAAACAGAGAGGTTGTTTCTGCTTAAAAACAACACACTTGGTGTCTGGGCCCACAGACTC
CTTTGCACTTATTCCACGTGTGACAGCCAATGTGCCTCGTTGCTTAGCAGACAGCATGTTACCGTCTTTC
CTGCTCAGTTTGTTAGCTCTATGGAATGGAATTTATAATCAATGCCCATACCAACATTTCACTAATATCA
TAGGAGATTTAGTCTCCATCTGGGTGTACATTACATTTGCTCTGGGGTGCTCCAGGCTGGGGGGTTGCCA
AGGAAGAGAAGAGAAACCGCAGAGAAGACGGGAGGGCAGGGCAGGGGTCTCTGAGAAGGGGAGGGGTCCC
AGAGTGCAGGAGCAGGAGCCAGGCTCATGAAAGGGGCCACGGGCGGGAGTATCCAGGGACGGCAGTCAAG
ATGGAGCACAGCTTAGGAAGCTGAAGGGAATCCTGGCCCACCTGGGTGCTAGAGGGCACATAGGAAGTGC
AGGAAGCAGACCAAGGTCCCCAAGAGAGGGAGACCTGGACGCTGAAGCATTTTCTGTCTTTATTAAGACA
ACTCCGTAAGAATTCCTGCTGGGCCAAAGTGAATTCTAGGATGCGACTTTAAGATGGGAGCAAGCGAACC
ATTGAGGAGGCAGGTTACCCTAGTTAGCCAATGCAGATCGAGAATGGGAAATCTTTCATTTATTCATGCA
ACAGATATTTAACGAAGCCCTGCCGTGTTCCAGGCCTGTGATAGATGCTGGAACAGGTACAGAGATACAG
GTGTCATTAATTGATCAGGGCAACCTCTCCTTCTGAGTCTTGCTGGAGCTTCAGATGCCCCTCACACAGA
GCTCGAGGGAGCCTCAACAATTGATCAGAAGTCAGGCACCATGGCTCACGCATATAATCCCAGCACTTTG
GGAGGCCAAGGCAGGTGGATCACTGGAGCCCAGGAGTTCCAGATCAGCTGGGGCAACATGGCAAAACCCC
ATCTCTATTAAAAAAAAAAAAAGTAACTGGATGTGATGGTACACACCTGTAGTCCCAGCTACTTGGGAGG
CTGAGAGGTGGGAGAATTGCTTGAGCCCGGGAAGTCGGGGGTCCAGTGAGCCTTGATCACACCACTGCAC
TCCAGCCTGAGTGACAGAGCAAGACCCTGACACACACACACACACACACACACACACACACACAGATTAG
AGCTGAAACAGGAGTAGAAACCTATCTGTATCTCTGATGAGATCAGATCTTTCTGATGAACAGAAAGAAT
GTAACCCCTGTACTCACACCCTCTCTGCTGGTTACATATGTTAACACGATTTCTCAAATGAGGCTTTTGG
TTGCAAATAAGAGAAAATCACTCACGCTGGCCCTGTGTTTTTCAAATTGTTTATTGTGATCAACATTTGA
AAAAAGAGCCGAGACTCTCAAGAGTGCATTACCCACGGTAAGGGTGAATTTTACTTCTTGACACTTATTT
CTCTTACATGTATCTATCTGTCTCAAATGAAAAATATATTTAGAAAGTTGAAAGCTATCCAAGTGAGTAT
AAGAAAAGAGTATCTCACCCTGAAGGCTAAGGACAGGGAGGGCCACCAGGCCTCACGAGGACCCAGGAAC
CACAAAGAAGGCTAGGAAGGAGCACAGGCGGTGACCATACTCTGGCTCAGTGGCTATGTGGGCTCTGGTC
TCTCTCAGCTGTTCCATGCATATGAGGCCAAATGTGGCTACCCTAGAGCTTCTGAGCCCTCAACAGAGAT
GAACTGGACTCTCTGCAGCCCCACTCTAAATTCCTAAGAGAGAAGTTGATTGACCCAATCAGGGTCAGGA
GAAGGAAGGGAGGAGGAAAGGGAGGAGAGAAGAGCCTCTTCGTCTCTTGCCTACCACTGGCCAGGCAATT
GTAGCCAAGGGGGCTGGAGTGTAAATGCAAACATAGCCATCAAGGGTTGTGTATGTGTGTGTGTGTGTCT
GTGTGTGTGTATGTGTGTCTCTTGGGTAGGTTAGATCTCCCAGGAGGTCCCTACTAAACAGACTTAAGCC
CGCAAAATTTTAGCTCTCCAGCCTCACACACTCCACCCCTCTACCATATTGAATCTTCCCAAACCAACTA
TGGCTTTCCCTAACTCCGGAGCTTGGCCTGGAATGCCCTGCTTCCCCTCTTTCCCCTGGGGAACGCCTGT
CCTTCAGGCCTCAGTTCACACACTGCCTCCCTTGCAAAGCTCTCCTCCCATCCCCGGAGTCCCTCTTCCC
CTTTGTTCTTTGGGTTCTATGCTTCTTCCCTCATAACTCCCACCAGGTTGTGTTAAAATGAGTTGTTCAA
GGTCCTGTCTGTTCCACTAGATTCTGAGCAACTTGGAGAACGAAGATCCAAACTTCGCTGCCTTTATTTC
CTCCTTTGTTCTTTTCTCATCCCCAAGTCCCTTCCAACTTGGAGTTATGAAGAAAGGAAGGAAGGAAGGG
TGGGAGGGAAGAACAGGAGGGGATCCCACAGGAGAATGTGTATAGGGAGAGGACTCAGACTAGCTAAAGC
TTTTCCCTCATAATTAATAGCAAATACCATGTTACCTGAATTTAATTCACAGTAGCATACAAAAGACTCG
CTTTGTTCTCCCCATTGATGTCATCAGAGGGCTGTGGGCAGGCCTAATCTTGGCTCAGGAGGCCCTCCAG
CCTGGATCTAAAGAGCAGCAGATGGGCCAGGCTCGGTGGCTCATGCCTGTAATCCCAGCATTTTGGGAGG
CCGAGGCGGGTGGATCACGAGGTCAGGAGTTTGAGACCAGCCTGGCCAAGATGGTGAAGCCTCGTCTCTA
CTAAAAATACAAAAATTAGCCAGGTGCGGTGGTGGGCGCCTGTATTTCCAGCTACCCGGGAGGCTGAGGA
GGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGGTCACGCCACTGCAC
TCTAGCCTGGGCAACAGAGCAAGACTCCGTCAAAAAAAAAATAAAAAAATAAAAAAATAAAAAAAATAAA
GAGGAGCACACATCTCTGCCCATCCTAACTCCCACTTTGACATTGAGGTCCCCAGGATGGAGGGTCTGCC
TCCATCTGCCTTGTCCCCTGCAATGGTGGGAAGGTGATGGAGCTCAAGTCTAGAGGCCACCAGCTTCTTA
GGGAGGTAGGAGGTGGAGGGTGGGGTGCGGGCCCTGCACACAACTGCCAAGTGAGGATGGGGGTGGGGTC
CACCTGAGGATAAGTAACAGTGAGGCTGGTGCAGAGGACCCAGGTGGAGGTAGACAGCAGAATTTGTGGT
GGGGTGGATGGCACATTATATAAGCCTCTCTTGCTGCCCTGTTTACTGAGATTGTTTCATTATCTTTTTT
GGCTTTTGTTTTTAAGAGATGGGGTCTTGCTGTGTCACACAGGCTGGAGTGCACTGTGTGATCATACCTC
ACTGCAGCCTCGACATCCTGGGCTCAGGCAAACCTCCCACCTTGGCCTCCCAAGTAGCTGGGACCACAAG
CGTTTGCCACCACACTCAGCTATTTTTATTTTTATTTTTATTTTTTTTAGAGATGGGGTCTTGCTGTGTC
GCCCAGGCTGGTCTTGAACTCCTGGGCTCAAGCGATCCTCCTGCCTTGGCCTCCCAAAGCCCTGGGATTA
TAGGCTGAGCCACCACACCCAGCCACATTTCATCTGTGCAGCTCCAGGGGCTCCACATTCTACTCTTCTC
ATTTCTTCTCCAGGGTACCCATGGCAAGGGATGAGGGTAGAAGATGGGGCAGCCAGGCCTTGATTAAAGG
AGAAGGAAGGCAGCCTGTGGAGAGGGCAGCCCAGGGAGTGCAGAGAGAAGTGGGCCATGAGGGAGACAGC
AGAGTGCAGGCTGCGTCCCAAATGAGCATACAGCCCACTGTGAGCCCACCATCTTCCTAGAGACCCCTCT
CCTCTCCAGGAGCTGCTTCAGTAGCACTCAGAGGAAAGAATGATGCTGTATCAACATTTCAGCAGCTCAT
CTTTTAACTCTAAGAAAATGGCAGCTCCTAAATGTTCAAAACTGCTTTGGAAACTTCTGGAGAGAGGTTT
TGCAGCTCAGGCAGACAGCTGATCGCGGCCTTTCTTCCACCCCAACCCATGCTCTCCCCATGCTCTCCTG
CCACAGCTGCAGCGGGCCCCTGGGTCCTACATTTGCAGCCCTTTGTCTCTGAGCTCAGACTTCCAATTCC
AAGCGGCAGCTGGGCAGGCTCACCAGCATGTCCAGCCAGTACTAGGACATCAGCAGGAGCCCAACCACCT
CTTTCCAAAATCTCTCCTCATGTCTCTCCTAGTTTCCATCTCCATCCTTCTAGTCAGCCAGGCTGAAAAC
ATTTGCTCCTCAGGGTGCAGAAGGGAAAGCTTTGCCTCCCTTCCTGGTGCTCACTGCCCCTGCGATTCCA
GCCCAAGCCCTCCCCGGCTCCTCACCCTGGTGTCAGCTGGAAGCCACCATCTCCTAAACCCACCTGTGTT
CTTCCACCTCTGCCAGGGCTGCCCTCTCCTCCACCTTCACAAACTCAATTCCTACCCATTCTCAGGTCCC
TTATCAAATGCCATCTCCTCCATGATGCCTCCCTGATTCCCCTGCTGGAAATAATGGTGATAACAGCTAA
GGCATTGGGGTTGGCTACGTGCCAGGCAAGGAGTTGGCACTTTACATGCTTTATCTCATTTCAGCCACAT
AACATCGACAGGTGGCATTATGATTCATATCATCCCCATCTGATAGCCAGGAAAACTGAGTCCCAGAGAG
GTTAGCCACTTTCCTAGGGCCCTGTGCTCTGACTCAAGCATAGCTCTGAGGAACTCTAGCATTCATCAGT
TTAAGCACCATGACTTTCTTTGCTGAGTCACCCAAGGCATTTCTTCATTTAAATGTTCTTCCTTGGCCAG
GCGCAGTGGCTCAGGCCCAATGCGGTGGCTCACGCCTGTAATCTCAACACTTTGGGAGGCCGAGGTGGGC
AGATAATCTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATA
CAAAAAAATGAGGCTGGGCGTGATGACTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGTGG
ATTACATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAATCCTATCTCTATTAAAAATACA
AAAAATTAGCCAGGCATGGTGGCAGGCACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAAAATGG
CTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAAGGTTGCACCATTGCACTCCAGCCTGGGCAAAAAGA
GGGAAACATCGTCTAAAAAAGAAAAAAAAAAAATTAGCCAGGCTGGGTGGTGCATGCCCGTAATTCCAGC
TACTCAGGAGGATGAAGCAAGAGAATTGCTTGAACCCAGGAGGCAGAGATTACAGTGAGCTGAGATCACA
ACACTGCACTCCAGCCTAGGTAAAGAACAAGACTCCATCTCAAAAATAAATAAATAAAAATAAATGTTCT
TCCTTGCAATGAAGTTAAATATGTAAATTCTCAAACCAGTTGCTTAAGGGCACAGTTTTGTTCTTTACCT
ATATTTTTAACAAATATTTTATGTAAGTAGTTGACAAAATCAAATACTGTGTACACTACCGAGGCTTCCC
TGGGAAAGCCATCAGCCTCTGCCCCATCCCTTCCCACTCCTGATTCCACTTTCCTGTGTTTCCATATCTT
TTTCATGTCTGTTTCTGGCCCACAGTGGGCGATCAATACATGTTAGCCACCAACCATCAAACCTATATTG
AGTAATTATGGTATGTCAGGCACTATGCTCAATGAAATTGTATTAGGCTTGTACAAAAGTAATTGTGGTT
TTTAAGAGTAATGGCAAAAACGGCAGTTACTTTCGCACCAACTATTTGCTGCCTTGAATTATTCCTCCTC
TCCTCATCCCTAAACCCTGCTCCTCCCAGCCATTCTTCCTCCCCTTCTTGGGCCATGGCCAGGCCCCACC
CAGGTACTAAGACTCAGGTGAACCAAGGAAGACTTAATGCCCACTCTTTTCTGATGCCCATGTTGGCATG
TGTTAAGTCGGTTAGCATTAAGTTTGGCTGCATTTAGCAGAGACCCAAAAGAACAGTGCCTTTTAAAAGG
CAGAGGTTATGTCTCTCACACACACCCAGCACAAGTCCAAGACCAGCATGGCATCTCAGCTCCATCAACC
TCAGGAACCGAGCTCCTGCAGCTCCCTGCCCTGCAGTTGATAAGGTGAGGTCTTTGTCCTCCTGGTTCAA
GATGGTGCTAGAATGTTGGCTACCATATCTATAGTCCAGGCATCAGAATGGAGCAAGGGATGAAAAAGGA
AGAGATGAAGGCACACGACAGGTTCCTGAGAGCTGGCACAGGACACTTCTGCTTATATTTCACTGGCCAG
AACTTAGTCACATGGTCACACCTAGTTGGGAGACTCTGAGAAGTAAAGTATTTATTCTAGATGGCCATAT
CCCTACCTAAGACTTGGAGTTTTCTATGACTGGGGAAGAACGGAAGACAAGATATTGGGAAAGACTAGCA
GCCTCTACTAAAAGGGTGATCTGTGTTGATGTGCGTGTGTGTGTGATGTTTGTATGAGCATGTGTGTTAT
GTGTTGTGTGTTGGTGGGGCAGATTCTTGCGAGCACTTTGGTCTCAGATGGACCTGCTACCAGTTCTCTC
TGCAGACCCCCATAGGTTTCTCCTAAACCTGGCCTCTCCTATTAGGCAGCCTTACTCAGCGGCAGCTTCT
CAGCTCCATGTTTTCAAGGAACCACAATTTATTTCCAGCATCCACTGAAGCATATTATCAGTGGTGATAG
AGGGGGCTTGTAAAACTGTTTTTCCACTTAGGTATTAGAGGGTGGCCATTATTTGAGAGTGACTATGACC
ACAGTTAATCTGGTAATAAATTCTCTTGGGTAGGAGGAAAGGAAAGGATGCTTTAAGGAAGCATCTTGCC
AGGAGACACAAAGCTAACAAGAGTGGAGCCTGCAGCTGGAGCCGCAGAGCCTAATCACTACACCCGCCCA
TCTCTGCTAGGGTTTCATGACTTCGTATCGGGGATTAGCAGTATTTAACTCTGTTGCACAAACATTTGGT
GTATTATTCAGGTAACAAGTAGCTAATAGAGGAAGTTTTACTTTTTTAAGACATAAATTTGCCTTTTCCC
AAATTACTTGGTACATAGTACTTTTCATGTTTGAAGTTGAGATGTGGGTACAATACCATAGCTTTATTCC
AGAGCAGGGTATTTGTTTCCAAATGCCATGTTCCCAGCAGCTGCCCTTGACTGGGAATTGGGGTGTGATT
TGGGCTTTTCCTTAAATCCTTGAGGAGCTGGAGGGGTGGGTGGCTCGCACTCCTGCTTTCTGGATCTGAA
TCCTGACTCTGTCATGGACCTGTTTGACTTTGGGCAAGTTGACTCCTATTCCTGAGCCCCATATTTTTCT
CTTCTGTGAAATTCAGATTAAAAAAACATGGCTTTGATCAAACATTATAAATAATATATAGACAGACTGC
TTGTTTTTATTGTATTGCCAGAAATGAATCCTACTAATATTGCCATCTATGGACAGAAAATGTATTACCT
GTCTTCATCAAGACCCAGACGAGGAAGAACACGAAAAGCGGAGATTAATTTTACTGCCATCTCCAGAACC
GTCATCCTAATATTTACTTACATTTTATTATTATTTCAGGCTCATGCACATATACTTAGCATGGATCATT
GGCCACAGACTCGCATACATTTAACTTTATTACCTTTTGCCTCATGTATCTCATTAAAATTTTGCTGCTT
AATCAAGGATCTGCATATTATTTTAATTTTAGAATTCACAGTTCCAAGACTTTGAAAGTTTCAAGCGTTC
TGGGTGAATGTGTTATGCTCTCTCCCGCCACCATGTCTTTATACCCCCTGATTTCTCAGCCACTATGGCA
ACCACTTTCTACTCTTAGTAGCCCATATTTAGTCCAATCCCCAGCTCAGGAGACACTTCTTCCAGGGAGC
CCCCTGTGCCTTCCAGTAGTATCTTGTACCTGCCCTTTTTGCAAAGCTCTTTCCTCCTGGCTTAGAATGG
CCCATTGACCTGTTTGTTTCTCCTATTAAACTGTAAGCCACTCGAGGGTAGAGAGCATCTGTTGTTCACC
ATTGCATCCTCGGTGCTGAGCACTGCGTCTGACATATTATTTAGAAGGTCAGTAAGTGCTAGTGGGATTC
AGGCTCCCAGTGGGTGGGAGAGAAAGGACGTAAGGAAGCAAGTGGTAAAGGCCCTCACAGAGTATCAGCA
GGCTGGTGTGAGGGAGAAATGCAGAGGATGGGTGAGTAGCATAATCGCTAATGATAGGGTAATGATAGAG
CACATTTCACAACACCTTTAAGCCCTTTCACGTGCATCAGATAATTTGATCCTCATAAAAGCCTAGAGAT
AGATATATTACAGGGATGAAGGTGGAGTATTTTGTGGTTATGTGATATGTTTAAAATTATGCAGTGAGTA
AATGACTGGGTTCAAACCAGACCTTAAAAGTCTGTTATCTTTCCCTCGAGCATGCAATGAAGTCTACATC
ATCCCTACCATGTCCATTTGATCACACCCTGGCCTCACAGCTCTGTGGTCTACAGGATACCTCATGGTGG
TTTTATTGACCAGACAATAATCCTCTTTCTAAGGGGATGCATTTCATTAATACATATGTAGATCATGAAT
TGTCTTTGACTTTGAGGGGATGGTAGCCAGAGCAGAAAGCAAAGCTGATTTTCATCCCCGTCTGGTAATG
TGGTTGGTAATGTGAAGATGGGTGTATTCTGAGATACCGGCTCCTTGCAGTGTGTGGTTCCTTCTGTTTT
CAGGCCCAAGAAGCCCATCCTGGGAAGGAAAATGCATTGGGGAACCCTGTGCGGATTCTTGTGGCTTTGG
CCCTATCTTTTCTATGTCCAAGCTGTGCCCATCCAAAAAGTCCAAGATGACACCAAAACCCTCATCAAGA
CAATTGTCACCAGGATCAATGACATTTCACACACGGTAAGGAGAGTATGCGGGGACAAAGTAGAACTGCA
GCCAGCCCAGCACTGGCTCCTAGTGGCACTGGACCCAGATAGTCCAAGAAACATTTATTGAACGCCTCCT
GAATGCCAGGCACCTACTGGAAGCTGAGAAGGATTTGAAAGCACAGGGCTCCACTCTTTCTGGTTGTTTC
TTTTGGCCCCTCTGCCTGCTGAGATTCCAGGGGTTAGTGGTTCTAATTCTAAACCACTCCAAGAACATTT
GATTTTGCTACATGTTTCCATTTAAAAATCATAGGATTTGGGCTGGGTGTGGTGGCTTGTACCTGTCATC
CCAGCACTTTGGGAGGCCAAAGCAGGAGGATCATTCGAGCCCAAGAGTTCGAGACCAGCCTGGGCAGCAT
AGGGAGACCCCATCTCTACAAAAATAATAAAAAATGTTAGCTGGGCATGGTGGTGTGTACCTGTGGTCCC
AGCTAGGGGAGGCTGAGATGGAAGGATCACCTGAGCCTGGGAGGTTGAGGCTGCAGTGGGCCCTGATCAT
GCCACCGTGCTCCAGCCTGGGTGACAGAGTGAGACCTTGTCTCAAAATAAATAAATAAATAAATAAAAGT
CATAGGATTTGATCAGGCATGATGGGTCACATCTGTAAGCCCATTGCTTTAGGAGGCCAAGGTAGGAGGA
TCAGTTGAGGCCAGGAGTTCAAGACCAGCCTGGGCAACATGGCAAGACCTCTCTCTCTAATTTTTAAAAA
AATAAAAATTAAAGATAAGAAAAAAATCATAGGATTCTCATGAGGCCTCACGTGCTTATTTTCAACCTAC
CAAGGGGAAACCCAGGCCTCAGCGATTAGCTGAGCCACATGCAGGCACAGCCACTGTCTCTTTCCTTCCT
GTCCCCTCTGTCCCCACCTTCTGCGCTCGCCTTCCTCCCTGACTTCACTTCCTTGAATCTTAGTGCCTAC
GACCAGAGGGAGCTGTGAAGTTCCTTGTGTCCCATTGGCAGGAACAAGACCCCCAGAAGCATCTCCTCAG
GGCCTCTATCCCATCTCTAGATGTGCTTGTCATTAGGGTTCTTGTAGTTCCAGCTGATCTCTGGCCCTGC
CGCTCAAAGATACCCAAAAGAGCGAGTCTACCCTTTTTCACATTCAACCCTCTACTGATTTGCAAATAGC
AGTCAGTGCCCACCCTGGTCTTTTCTCTGGGGTCCAGCAGGCCTAGACCTTCAGCCATTTTCCTGATGAG
GTCTGTATTTGAAATTAGGAAGATTAAGTTTGAATCTTCACACTTCTGATGTCTGTGAGATCTTCAGCAA
GTTCCTTACTGTCTTTAAGCCTTGTTTTCATCATCTGGATAATGGGGATATCACACACTATTCACAAGGT
TGTTATGAGGCCTAAATTAGCTAAAGCAATTGAATCCTCCTTACCCCCTGCATGGAGCTCTCTGGAGACT
TCCACGTCTCCTGGTCATTGTGGGTGTCTTATGGTAGTCTTGGGCAGTTAGGGAGAAGTTAGGTGTCTGG
AAGCAAAGATGGCTCAGAACTAGATAGAGTCTTGGGCATTTTATAGATAAAAACTCTTGTCTCCTTTAAA
AATAATAAAAAAAAATTAGCTGGGCATATTAGCCACTCAGCAAGACTGCACGTGATAGATCCCGAGTGCC
CCACCTTGGGTGGTGTAATACACAATATCACGGGAGCCCCGGGTAGTAACCACGGAGGTGTCAGCCTCAG
TGCTGTGGGCAGATGGATGGGGAGAGCCTCCCGGAACTGGAGTCACTGGAGCAGGGTTGGGGGGCCTCAC
TGAGGGTACGGCCTTGATCTCTAAGGAGGAGGGACTGCCTGGAAAAGCTGACTGGGAGGGAGGACTCGGC
TGGGGGTAGAAGGGACTAGGGAAGGCTGGGGGTGGGGGTGCTTATGGAGGACCTCAGATGCCTGGGGAAC
AGACTCCACTAAATAAAACATATGAAACCATGGCTGGTTCTTCAGCAGAGGCCATGTAGAGAAAGGAATG
ACCTAGGAAAGTTGGCCTGGAAGTGGAGGGAAGGATGGTGTGGGAAAAGCAGGAATCTCGGAGACCAGCT
TAGAGGCTTGGCAGTCACCTGGGTGCAGGATACAAGGGCCTGAGCCAAAGTGGTGAGGGAGGGTGGAAGG
AGGCAGCCCAGAGAATGACCCTCCATGCCCACGGGGAAGGCAGAGGGCTCTGAGAGCGATTCCTCCCACA
TGCTGAGCACTTGTTCTCCCTCTTCCTCCTGCATAGCAGTCAGTCTCCTCCAAACAGAAAGTCACCGGTT
TGGACTTCATTCCTGGGCTCCACCCCATCCTGACCTTATCCAAGATGGACCAGACACTGGCAGTCTACCA
ACAGATCCTCACCAGTATGCCTTCCAGAAACGTGATCCAAATATCCAACGACCTGGAGAACCTCCGGGAT
CTTCTTCACGTGCTGGCCTTCTCTAAGAGCTGCCACTTGCCCTGGGCCAGTGGCCTGGAGACCTTGGACA
GCCTGGGGGGTGTCCTGGAAGCTTCAGGCTACTCCACAGAGGTGGTGGCCCTGAGCAGGCTGCAGGGGTC
TCTGCAGGACATGCTGTGGCAGCTGGACCTCAGCCCTGGGTGCTGAGGCCTTGAAGGTCACTCTTCCTGC
AAGGACTACGTTAAGGGAAGGAACTCTGGCTTCCAGGTATCTCCAGGATTGAAGAGCATTGCATGGACAC
CCCTTATCCAGGACTCTGTCAATTTCCCTGACTCCTCTAAGCCACTCTTCCAAAGGCATAAGACCCTAAG
CCTCCTTTTGCTTGAAACCAAAGATATATACACAGGATCCTATTCTCACCAGGAAGGGGGTCCACCCAGC
AAAGAGTGGGCTGCATCTGGGATTCCCACCAAGGTCTTCAGCCATCAACAAGAGTTGTCTTGTCCCCTCT
TGACCCATCTCCCCCTCACTGAATGCCTCAATGTGACCAGGGGTGATTTCAGAGAGGGCAGAGGGGTAGG
CAGAGCCTTTGGATGACCAGAACAAGGTTCCCTCTGAGAATTCCAAGGAGTTCCATGAAGACCACATCCA
CACACGCAGGAACTCCCAGCAACACAAGCTGGAAGCACATGTTTATTTATTCTGCATTTTATTCTGGATG
GATTTGAAGCAAAGCACCAGCTTCTCCAGGCTCTTTGGGGTCAGCCAGGGCCAGGGGTCTCCCTGGAGTG
CAGTTTCCAATCCCATAGATGGGTCTGGCTGAGCTGAACCCATTTTGAGTGACTCGAGGGTTGGGTTCAT
CTGAGCAAGAGCTGGCAAAGGTGGCTCTCCAGTTAGTTCTCTCGTAACTGGTTTCATTTCTACTGTGACT
GATGTTACATCACAGTGTTTGCAATGGTGTTGCCCTGAGTGGATCTCCAAGGACCAGGTTATTTTAAAAA
GATTTGTTTTGTCAAGTGTCATATGTAGGTGTCTGCACCCAGGGGTGGGGAATGTTTGGGCAGAAGGGAG
AAGGATCTAGAATGTGTTTTCTGAATAACATTTGTGTGGTGGGTTCTTTGGAAGGAGTGAGATCATTTTC
TTATCTTCTGCAATTGCTTAGGATGTTTTTCATGAAAATAGCTCTTTCAGGGGGGTTGTGAGGCCTGGCC
AGGCACCCCCTGGAGAGAAGTTTCTGGCCCTGGCTGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCT
TCAAATCCATCCAGAATAAAACGCAAAGGGCTGAAAGCCATTTGTTGGGGCAGTGGTAAGCTCTGGCTTT
CTCCGACTGCTAGGGAGTGGTCTTTCCTATCATGGAGTGACGGTCCCACACTGGTGACTGCGATCTTCAG
AGCAGGGGTCCTTGGTGTGACCCTCTGAATGGTCCAGGGTTGATCACACTCTGGGTTTATTACATGGCAG
TGTTCCTATTTGGGGCTTGCATGCCAAATTGTAGTTCTTGTCTGATTGGCTCACCCAAGCAAGGCCAAAA
TTACCAAAAATCTTGGGGGGTTTTTACTCCAGTGGTGAAGAAAACTCCTTTAGCAGGTGGTCCTGAGACC
TGACAAGCACTGCTAGGCGAGTGCCAGGACTCCCCAGGCCAGGCCACCAGGATGGCCCTTCCCACTGGAG
GTCACATTCAGGAAGATGAAAGAGGAGGTTTGGGGTCTGCCACCATCCTGCTGCTGTGTTTTTGCTATCA
CACAGTGGGTGGTGGATCTGTCCAAGGAAACTTGAATCAAAGCAGTTAACTTTAAGACTGAGCACCTGCT
TCATGCTCAGCCCTGACTGGTGCTATAGGCTGGAGAAGCTCACCCAATAAACATTAAGATTGAGGCCTGC
CCTCAGGGATCTTGCATTCCCAGTGGTCAAACCGCACTCACCCATGTGCCAAGGTGGGGTATTTACCACA
GCAGCTGAACAGCCAAATGCATGGTGCAGTTGACAGCAGGTGGGAAATGGTATGAGCTGAGGGGGGCCGT
GCCCAGGGGCCCACAGGGAACCCTGCTTGCACTTTGTAACATGTTTACTTTTCAGGGCATCTTAGCTTCT
ATTATAGCCACATCCCTTTGAAACAAGATAACTGAGAATTTAAAAATAAGAAAATACATAAGACCATAAC
AGCCAACAGGTGGCAGGACCAGGACTATAGCCCAGGTCCTCTGATACCCAGAGCATTACGTGAGCCAGGT
AATGAGGGACTGGAACCAGGGAGACCGAGCGCTTTCTGGAAAAGAGGAGTTTCGAGGTAGAGTTTGAAGG
AGGTGAGGGATGTGAATTGCCTGCAGAGAGAAGCCTGTTTTGTTGGAAGGTTTGGTGTGTGGAGATGCAG
AGGTAAAAGTGTGAGCAGTGAGTTACAGCGAGAGGCAGAGAAAGAAGAGACAGGAGGGCAAGGGCCATGC
TGAAGGGACCTTGAAGGGTAAAGAAGTTTGATATTAAAGGAGTTAAGAGTAGCAAGTTCTAGAGAAGAGG
CTGGTGCTGTGGCCAGGGTGAGAGCTGCTCTGGAAAATGTGACCCAGATCCTCACAACCACCTAATCAGG
CTGAGGTGTCTTAAGCCTTTTGCTCACAAAACCTGGCACAATGGCTAATTCCCAGAGTGTGAAACTTCCT
AAGTATAAATGGTTGTCTGTTTTTGTAACTTAAAAAAAAAAAAAAAAGTTTGGCCGGGTGCGGTGGCTCA
CGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGGGGATCACAAGGTCACTAGATGGCGAGCATCCT
GGCCAACATGGTGAAACCCCGTCTCTACTAAAAACACAAAAGTTAGCTGAGCGTGGTGGCGGGCGCCTGT
AGTCCCAGCCACTCGGGAGGCTGAGACAGGAGAATCGCTTAAACCTGGGAGGCGGAGAGTACAGTGAGCC
AAGATCGCGCCACTGCACTCCGGCCTGATGACAGAGCGAGATTCCGTCTTAAAAAAAAAAAAAAAAAAGT
TTGTTTTTAAAAAAATCTAAATAAAATAACTTTGCCCCCTG

Identify web Primer design

1. http://www.clcbio.com/index.php?id=493
2. http://dbb.nhri.org.tw/primer/
3. http://albicansmap.ahc.umn.edu/
4. http://bioweb.uwlax.edu/GenWeb/Molecular/Seq_Anal/Primer_Design/primer_design.htm
5. http://seq.yeastgenome.org/cgi-bin/web-primer
6. http://bibiserv.techfak.uni-bielefeld.de/genefisher/
7. http://www.exiqon.com/
8. http://www.cbi.pku.edu.cn/mirror/GenomeWeb/nuc-primer.html
9. http://www.caddprimer.com/
10.http://www.premierbiosoft.com/